Expression of Hemagglutinin of Haemophilus paragallinarum Serotype A in Escherichia coli.
نویسندگان
چکیده
منابع مشابه
Serological classification of Haemophilus paragallinarum with a hemagglutinin system.
Antigens, prepared from 17 strains of Haemophilus paragallinarum by treatment with potassium thiocyanate followed by sonication, uniformly agglutinated glutaraldehyde-fixed chicken erythrocytes and formed specific hemagglutination inhibition antibodies in rabbits. Attempts were made to classify the strains into serotypes by a combination of cross-hemagglutination inhibition and cross-absorption...
متن کاملProposal of a new serovar and altered nomenclature for Haemophilus paragallinarum in the Kume hemagglutinin scheme.
By using the Kume hemagglutinin serotyping scheme, 13 Australian isolates of Haemophilus paragallinarum were shown to constitute a new serovar within the presently termed serogroup II. Because of the likelihood that new serovars will continue to be established, we propose a rationalization of the nomenclature of the Kume scheme. Under this altered scheme, the three recognized serogroups I, II, ...
متن کاملComparison of hemagglutinin and agglutinin schemes for the serological classification of Haemophilus paragallinarum and proposal of a new hemagglutinin serovar.
Ninety-five isolates of Haemophilus paragallinarum were classified serologically by a hemagglutinin serotyping scheme, and the results were compared with results from agglutinin serotyping of the same isolates. Of the isolates, 65 were from Australia, 5 were from Japan, 6 were from the Federal Republic of Germany, 7 were from the United States, and 12 were from South Africa. Seven hemagglutinin...
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Activin A is a member of the transforming growth factor β super family. Because of its extensive clinical usages, its recombinant production is beneficial. In this study, activin A was expressed in E. coli using the pET 21a expression vector. The optimization of the activin A production in E. coli was done by using the response surface methodology (RSM). At this stage, the effect of IPTG and la...
متن کاملmolecular cloning, expression and purification of truncated hpd fragment of haemophilus influenzae in escherichia coli
conclusions our results showed that the recombinant protein produced by the pbad vector in the escherichia coli system was very efficient. background nontypeable haemophilus influenzae (nthi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. protein d (pd) belongs to the minor outer-membrane proteins of h. in...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 1991
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.53.917